Lab

Peripheral Smear

search

Peripheral Smear, Peripheral Blood Film, Peripheral Blood Smear, Blood Film

  • Technique
  • Staining
  1. Staining
    1. Avoid water contamination
    2. Romanowsky variants based on methylene blue and eosin
      1. Wright Stain (Sodium Bicarbonate)
      2. May-Grunwald-Giemsa Stain (acid bichromate)
  2. Slide evaluation
    1. Evaluate for adequate sample under 10x power
    2. Cell exam under oil immersion lens at 50x or higher
  • References
  1. Lee (1999) Wintrobe's Hematology, Lippincott, p. 23-25