- Clear Tape (e.g. Scotch Tape)
- Avoid Magic tape or Package Tape
- Flashlight
- Assistant to spread buttocks apart
- Perform immediately after awakening
- Repeat test on 3 consecutive days
- Roll tape around 4 fingers of hand, sticky side out
- While assistant spreads buttocks
- Identify involved perianal area with flashlight
- Touch involved perianal area with tape 1-2 times
- Place Tape on a slide with clean side down
- Trim tape, label slide and bring to clinic
- View slide under direct microscopy
- Test Sensitivity: 90% if done on 3 consecutive days